cdna synthesis kits Search Results


cdna synthese kit  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd cdna synthese kit
Cdna Synthese Kit, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdna synthese kit/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
cdna synthese kit - by Bioz Stars, 2026-05
90/100 stars
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cdna synthesis and library preparation kits  (Celemics Inc)
90
Celemics Inc cdna synthesis and library preparation kits
Cdna Synthesis And Library Preparation Kits, supplied by Celemics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdna synthesis and library preparation kits/product/Celemics Inc
Average 90 stars, based on 1 article reviews
cdna synthesis and library preparation kits - by Bioz Stars, 2026-05
90/100 stars
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cdna synthesis kits  (Beijing Genomics Institute Shenzhen)
90
Beijing Genomics Institute Shenzhen cdna synthesis kits
Cdna Synthesis Kits, supplied by Beijing Genomics Institute Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdna synthesis kits/product/Beijing Genomics Institute Shenzhen
Average 90 stars, based on 1 article reviews
cdna synthesis kits - by Bioz Stars, 2026-05
90/100 stars
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kits for cdna synthesis and real-time pcr (rt-pcr) detection  (Yeasen Biotechnology)
90
Yeasen Biotechnology kits for cdna synthesis and real-time pcr (rt-pcr) detection
Kits For Cdna Synthesis And Real Time Pcr (Rt Pcr) Detection, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kits for cdna synthesis and real-time pcr (rt-pcr) detection/product/Yeasen Biotechnology
Average 90 stars, based on 1 article reviews
kits for cdna synthesis and real-time pcr (rt-pcr) detection - by Bioz Stars, 2026-05
90/100 stars
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first strand core dna (cdna) synthesis kits  (Promega)
90
Promega first strand core dna (cdna) synthesis kits
First Strand Core Dna (Cdna) Synthesis Kits, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/first strand core dna (cdna) synthesis kits/product/Promega
Average 90 stars, based on 1 article reviews
first strand core dna (cdna) synthesis kits - by Bioz Stars, 2026-05
90/100 stars
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cdna synthesis kits  (PCR Biosystems Ltd)
90
PCR Biosystems Ltd cdna synthesis kits
Cdna Synthesis Kits, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdna synthesis kits/product/PCR Biosystems Ltd
Average 90 stars, based on 1 article reviews
cdna synthesis kits - by Bioz Stars, 2026-05
90/100 stars
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prime script ii 1st strand cdna synthesis kits  (Sangon Biotech)
90
Sangon Biotech prime script ii 1st strand cdna synthesis kits
Prime Script Ii 1st Strand Cdna Synthesis Kits, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prime script ii 1st strand cdna synthesis kits/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
prime script ii 1st strand cdna synthesis kits - by Bioz Stars, 2026-05
90/100 stars
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reagent kits for cdna synthesis and quantitative reverse transcription pcr (qrt-pcr)  (Bioneer Corporation)
90
Bioneer Corporation reagent kits for cdna synthesis and quantitative reverse transcription pcr (qrt-pcr)
Reagent Kits For Cdna Synthesis And Quantitative Reverse Transcription Pcr (Qrt Pcr), supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reagent kits for cdna synthesis and quantitative reverse transcription pcr (qrt-pcr)/product/Bioneer Corporation
Average 90 stars, based on 1 article reviews
reagent kits for cdna synthesis and quantitative reverse transcription pcr (qrt-pcr) - by Bioz Stars, 2026-05
90/100 stars
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revertaid tm first-strand cdna synthesis kits  (Changsheng Bio Technology Co)
90
Changsheng Bio Technology Co revertaid tm first-strand cdna synthesis kits
( A ). The specificity analysis results of the multiplex RT-PCR detection method. The detection samples corresponding to lanes 1 to 7 only contain WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV, respectively. ( B ). The sensitivity tests of the multiplex RT-PCR detection method. The drop-out experiments were carried out to test the specificity of this multiplex RT-PCR, in which one pair was removed at a time to see whether the rest of the primers had cross-reacted.; lane 1: healthy plant (negative control); lane 2: detection of MYSV; lane 3: detection of PRSV; lane 4: detection of TMV; lane 5: detection of SqMV; lane 6: detection of ZYMV; lane 7: detection of CMV. ( C1 ). Detection results of using different Mg 2+ concentrations in multiplex RT-PCR amplification system. lane 1: at 1.0 mol/L; lane 2: at 1.5 mmol/L; lane 3: at 2.0 mmol/L; lane 4: at 2.5 mmol/L; lane 5: at 3.0 mmol/L; lane 6: at 3.5 mmol/L; lane 7: at 4.0 mmol/L. ( C2 ). Detection results of using different template <t>cDNA</t> volumes in multiplex RT-PCR amplification system. lane 1: at 0.5 μL; lane 2: at 0.75 μL; lane 3: at 1 μL; lane 4: at 1.25 μL; lane 5: at 1.5 μL; lane 6: at 1.75 μL; lane 7: at 2 μL. ( C3 ). Detection results of using different primer amounts in a multiplex RT-PCR amplification system; lane 1: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 2: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 3: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 4: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 5: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 6: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 7: at 7 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1. ( C4 ). Detection results of using different dNTP concentrations in multiplex RT-PCR amplification system; lane 1: at 0.2 mmol/L; lane 2: at 0.4 mmol/L; lane 3: at 0.6 mmol/L; lane 4: at 0.8 mmol/L; lane 5: at 1.0 mmol/L; lane 6: at 1.2 mmol/L; lane 7: at 1.4 mmol/L; ( C5 ). Detection results of different amounts of Taq DNA polymerase in multiplex RT-PCR amplification system; lane 1: at 0.25 U; lane 2: at 0.5 U; lane 3: at 0.75 U; lane 4: at 1.0 U; lane 5: at 1.25 U; lane 6: at 1.5 U; lane 7: at 1.75 U. ( C6 ). Detection results of using different annealing temperatures in multiplex RT-PCR method; lane 1: at 50 °C; lane 2: at 51 °C; lane 3: at 52 °C; lane 4: at 53 °C; lane 5: at 54 °C; lane 6: at 55 °C; lane 7: at 56 °C. ( D ). The detection limits of the multiplex RT-PCR assays. The detection limits of this multiplex PCR were conducted by a series of sensitivity tests. The positive clone vector was adjusted to the same initial concentration and diluted serially ten-fold (10 5 to 10 10 copies/μL) to serve as a template in the optimized multiplex PCR. ( D1 ). The detection limits of WMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D2 ). The detection limits of CMV;1–6 stand for 10 5 to 10 0 copies/μL. ( D3 ). The detection limits of ZYMV; 1–6 stand for 10 5 to 10 0 copies/μL ( D4 ). The detection limits of SqMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D5 ). The detection limits of TMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D6 ). The detection limits of PRSV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D7 ). The detection limits of MYSV; 1–6 stand for 10 5 to 10 0 copies/μL. ”–“represents deionized water as control. M: DNA marker (100 bp–2000 bp).
Revertaid Tm First Strand Cdna Synthesis Kits, supplied by Changsheng Bio Technology Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/revertaid tm first-strand cdna synthesis kits/product/Changsheng Bio Technology Co
Average 90 stars, based on 1 article reviews
revertaid tm first-strand cdna synthesis kits - by Bioz Stars, 2026-05
90/100 stars
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fastking cdna first-strand synthesis kits  (TIGEN Inc)
90
TIGEN Inc fastking cdna first-strand synthesis kits
( A ). The specificity analysis results of the multiplex RT-PCR detection method. The detection samples corresponding to lanes 1 to 7 only contain WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV, respectively. ( B ). The sensitivity tests of the multiplex RT-PCR detection method. The drop-out experiments were carried out to test the specificity of this multiplex RT-PCR, in which one pair was removed at a time to see whether the rest of the primers had cross-reacted.; lane 1: healthy plant (negative control); lane 2: detection of MYSV; lane 3: detection of PRSV; lane 4: detection of TMV; lane 5: detection of SqMV; lane 6: detection of ZYMV; lane 7: detection of CMV. ( C1 ). Detection results of using different Mg 2+ concentrations in multiplex RT-PCR amplification system. lane 1: at 1.0 mol/L; lane 2: at 1.5 mmol/L; lane 3: at 2.0 mmol/L; lane 4: at 2.5 mmol/L; lane 5: at 3.0 mmol/L; lane 6: at 3.5 mmol/L; lane 7: at 4.0 mmol/L. ( C2 ). Detection results of using different template <t>cDNA</t> volumes in multiplex RT-PCR amplification system. lane 1: at 0.5 μL; lane 2: at 0.75 μL; lane 3: at 1 μL; lane 4: at 1.25 μL; lane 5: at 1.5 μL; lane 6: at 1.75 μL; lane 7: at 2 μL. ( C3 ). Detection results of using different primer amounts in a multiplex RT-PCR amplification system; lane 1: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 2: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 3: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 4: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 5: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 6: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 7: at 7 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1. ( C4 ). Detection results of using different dNTP concentrations in multiplex RT-PCR amplification system; lane 1: at 0.2 mmol/L; lane 2: at 0.4 mmol/L; lane 3: at 0.6 mmol/L; lane 4: at 0.8 mmol/L; lane 5: at 1.0 mmol/L; lane 6: at 1.2 mmol/L; lane 7: at 1.4 mmol/L; ( C5 ). Detection results of different amounts of Taq DNA polymerase in multiplex RT-PCR amplification system; lane 1: at 0.25 U; lane 2: at 0.5 U; lane 3: at 0.75 U; lane 4: at 1.0 U; lane 5: at 1.25 U; lane 6: at 1.5 U; lane 7: at 1.75 U. ( C6 ). Detection results of using different annealing temperatures in multiplex RT-PCR method; lane 1: at 50 °C; lane 2: at 51 °C; lane 3: at 52 °C; lane 4: at 53 °C; lane 5: at 54 °C; lane 6: at 55 °C; lane 7: at 56 °C. ( D ). The detection limits of the multiplex RT-PCR assays. The detection limits of this multiplex PCR were conducted by a series of sensitivity tests. The positive clone vector was adjusted to the same initial concentration and diluted serially ten-fold (10 5 to 10 10 copies/μL) to serve as a template in the optimized multiplex PCR. ( D1 ). The detection limits of WMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D2 ). The detection limits of CMV;1–6 stand for 10 5 to 10 0 copies/μL. ( D3 ). The detection limits of ZYMV; 1–6 stand for 10 5 to 10 0 copies/μL ( D4 ). The detection limits of SqMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D5 ). The detection limits of TMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D6 ). The detection limits of PRSV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D7 ). The detection limits of MYSV; 1–6 stand for 10 5 to 10 0 copies/μL. ”–“represents deionized water as control. M: DNA marker (100 bp–2000 bp).
Fastking Cdna First Strand Synthesis Kits, supplied by TIGEN Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fastking cdna first-strand synthesis kits/product/TIGEN Inc
Average 90 stars, based on 1 article reviews
fastking cdna first-strand synthesis kits - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
toprealtm first strand cdna synthesis kits  (iNtRON Biotechnology)
90
iNtRON Biotechnology toprealtm first strand cdna synthesis kits
( A ). The specificity analysis results of the multiplex RT-PCR detection method. The detection samples corresponding to lanes 1 to 7 only contain WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV, respectively. ( B ). The sensitivity tests of the multiplex RT-PCR detection method. The drop-out experiments were carried out to test the specificity of this multiplex RT-PCR, in which one pair was removed at a time to see whether the rest of the primers had cross-reacted.; lane 1: healthy plant (negative control); lane 2: detection of MYSV; lane 3: detection of PRSV; lane 4: detection of TMV; lane 5: detection of SqMV; lane 6: detection of ZYMV; lane 7: detection of CMV. ( C1 ). Detection results of using different Mg 2+ concentrations in multiplex RT-PCR amplification system. lane 1: at 1.0 mol/L; lane 2: at 1.5 mmol/L; lane 3: at 2.0 mmol/L; lane 4: at 2.5 mmol/L; lane 5: at 3.0 mmol/L; lane 6: at 3.5 mmol/L; lane 7: at 4.0 mmol/L. ( C2 ). Detection results of using different template <t>cDNA</t> volumes in multiplex RT-PCR amplification system. lane 1: at 0.5 μL; lane 2: at 0.75 μL; lane 3: at 1 μL; lane 4: at 1.25 μL; lane 5: at 1.5 μL; lane 6: at 1.75 μL; lane 7: at 2 μL. ( C3 ). Detection results of using different primer amounts in a multiplex RT-PCR amplification system; lane 1: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 2: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 3: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 4: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 5: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 6: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 7: at 7 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1. ( C4 ). Detection results of using different dNTP concentrations in multiplex RT-PCR amplification system; lane 1: at 0.2 mmol/L; lane 2: at 0.4 mmol/L; lane 3: at 0.6 mmol/L; lane 4: at 0.8 mmol/L; lane 5: at 1.0 mmol/L; lane 6: at 1.2 mmol/L; lane 7: at 1.4 mmol/L; ( C5 ). Detection results of different amounts of Taq DNA polymerase in multiplex RT-PCR amplification system; lane 1: at 0.25 U; lane 2: at 0.5 U; lane 3: at 0.75 U; lane 4: at 1.0 U; lane 5: at 1.25 U; lane 6: at 1.5 U; lane 7: at 1.75 U. ( C6 ). Detection results of using different annealing temperatures in multiplex RT-PCR method; lane 1: at 50 °C; lane 2: at 51 °C; lane 3: at 52 °C; lane 4: at 53 °C; lane 5: at 54 °C; lane 6: at 55 °C; lane 7: at 56 °C. ( D ). The detection limits of the multiplex RT-PCR assays. The detection limits of this multiplex PCR were conducted by a series of sensitivity tests. The positive clone vector was adjusted to the same initial concentration and diluted serially ten-fold (10 5 to 10 10 copies/μL) to serve as a template in the optimized multiplex PCR. ( D1 ). The detection limits of WMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D2 ). The detection limits of CMV;1–6 stand for 10 5 to 10 0 copies/μL. ( D3 ). The detection limits of ZYMV; 1–6 stand for 10 5 to 10 0 copies/μL ( D4 ). The detection limits of SqMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D5 ). The detection limits of TMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D6 ). The detection limits of PRSV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D7 ). The detection limits of MYSV; 1–6 stand for 10 5 to 10 0 copies/μL. ”–“represents deionized water as control. M: DNA marker (100 bp–2000 bp).
Toprealtm First Strand Cdna Synthesis Kits, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/toprealtm first strand cdna synthesis kits/product/iNtRON Biotechnology
Average 90 stars, based on 1 article reviews
toprealtm first strand cdna synthesis kits - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
first-strand mirna/cdna synthesis kits  (Cowin Biosciences)
90
Cowin Biosciences first-strand mirna/cdna synthesis kits
( A ). The specificity analysis results of the multiplex RT-PCR detection method. The detection samples corresponding to lanes 1 to 7 only contain WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV, respectively. ( B ). The sensitivity tests of the multiplex RT-PCR detection method. The drop-out experiments were carried out to test the specificity of this multiplex RT-PCR, in which one pair was removed at a time to see whether the rest of the primers had cross-reacted.; lane 1: healthy plant (negative control); lane 2: detection of MYSV; lane 3: detection of PRSV; lane 4: detection of TMV; lane 5: detection of SqMV; lane 6: detection of ZYMV; lane 7: detection of CMV. ( C1 ). Detection results of using different Mg 2+ concentrations in multiplex RT-PCR amplification system. lane 1: at 1.0 mol/L; lane 2: at 1.5 mmol/L; lane 3: at 2.0 mmol/L; lane 4: at 2.5 mmol/L; lane 5: at 3.0 mmol/L; lane 6: at 3.5 mmol/L; lane 7: at 4.0 mmol/L. ( C2 ). Detection results of using different template <t>cDNA</t> volumes in multiplex RT-PCR amplification system. lane 1: at 0.5 μL; lane 2: at 0.75 μL; lane 3: at 1 μL; lane 4: at 1.25 μL; lane 5: at 1.5 μL; lane 6: at 1.75 μL; lane 7: at 2 μL. ( C3 ). Detection results of using different primer amounts in a multiplex RT-PCR amplification system; lane 1: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 2: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 3: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 4: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 5: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 6: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 7: at 7 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1. ( C4 ). Detection results of using different dNTP concentrations in multiplex RT-PCR amplification system; lane 1: at 0.2 mmol/L; lane 2: at 0.4 mmol/L; lane 3: at 0.6 mmol/L; lane 4: at 0.8 mmol/L; lane 5: at 1.0 mmol/L; lane 6: at 1.2 mmol/L; lane 7: at 1.4 mmol/L; ( C5 ). Detection results of different amounts of Taq DNA polymerase in multiplex RT-PCR amplification system; lane 1: at 0.25 U; lane 2: at 0.5 U; lane 3: at 0.75 U; lane 4: at 1.0 U; lane 5: at 1.25 U; lane 6: at 1.5 U; lane 7: at 1.75 U. ( C6 ). Detection results of using different annealing temperatures in multiplex RT-PCR method; lane 1: at 50 °C; lane 2: at 51 °C; lane 3: at 52 °C; lane 4: at 53 °C; lane 5: at 54 °C; lane 6: at 55 °C; lane 7: at 56 °C. ( D ). The detection limits of the multiplex RT-PCR assays. The detection limits of this multiplex PCR were conducted by a series of sensitivity tests. The positive clone vector was adjusted to the same initial concentration and diluted serially ten-fold (10 5 to 10 10 copies/μL) to serve as a template in the optimized multiplex PCR. ( D1 ). The detection limits of WMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D2 ). The detection limits of CMV;1–6 stand for 10 5 to 10 0 copies/μL. ( D3 ). The detection limits of ZYMV; 1–6 stand for 10 5 to 10 0 copies/μL ( D4 ). The detection limits of SqMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D5 ). The detection limits of TMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D6 ). The detection limits of PRSV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D7 ). The detection limits of MYSV; 1–6 stand for 10 5 to 10 0 copies/μL. ”–“represents deionized water as control. M: DNA marker (100 bp–2000 bp).
First Strand Mirna/Cdna Synthesis Kits, supplied by Cowin Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/first-strand mirna/cdna synthesis kits/product/Cowin Biosciences
Average 90 stars, based on 1 article reviews
first-strand mirna/cdna synthesis kits - by Bioz Stars, 2026-05
90/100 stars
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( A ). The specificity analysis results of the multiplex RT-PCR detection method. The detection samples corresponding to lanes 1 to 7 only contain WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV, respectively. ( B ). The sensitivity tests of the multiplex RT-PCR detection method. The drop-out experiments were carried out to test the specificity of this multiplex RT-PCR, in which one pair was removed at a time to see whether the rest of the primers had cross-reacted.; lane 1: healthy plant (negative control); lane 2: detection of MYSV; lane 3: detection of PRSV; lane 4: detection of TMV; lane 5: detection of SqMV; lane 6: detection of ZYMV; lane 7: detection of CMV. ( C1 ). Detection results of using different Mg 2+ concentrations in multiplex RT-PCR amplification system. lane 1: at 1.0 mol/L; lane 2: at 1.5 mmol/L; lane 3: at 2.0 mmol/L; lane 4: at 2.5 mmol/L; lane 5: at 3.0 mmol/L; lane 6: at 3.5 mmol/L; lane 7: at 4.0 mmol/L. ( C2 ). Detection results of using different template cDNA volumes in multiplex RT-PCR amplification system. lane 1: at 0.5 μL; lane 2: at 0.75 μL; lane 3: at 1 μL; lane 4: at 1.25 μL; lane 5: at 1.5 μL; lane 6: at 1.75 μL; lane 7: at 2 μL. ( C3 ). Detection results of using different primer amounts in a multiplex RT-PCR amplification system; lane 1: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 2: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 3: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 4: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 5: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 6: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 7: at 7 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1. ( C4 ). Detection results of using different dNTP concentrations in multiplex RT-PCR amplification system; lane 1: at 0.2 mmol/L; lane 2: at 0.4 mmol/L; lane 3: at 0.6 mmol/L; lane 4: at 0.8 mmol/L; lane 5: at 1.0 mmol/L; lane 6: at 1.2 mmol/L; lane 7: at 1.4 mmol/L; ( C5 ). Detection results of different amounts of Taq DNA polymerase in multiplex RT-PCR amplification system; lane 1: at 0.25 U; lane 2: at 0.5 U; lane 3: at 0.75 U; lane 4: at 1.0 U; lane 5: at 1.25 U; lane 6: at 1.5 U; lane 7: at 1.75 U. ( C6 ). Detection results of using different annealing temperatures in multiplex RT-PCR method; lane 1: at 50 °C; lane 2: at 51 °C; lane 3: at 52 °C; lane 4: at 53 °C; lane 5: at 54 °C; lane 6: at 55 °C; lane 7: at 56 °C. ( D ). The detection limits of the multiplex RT-PCR assays. The detection limits of this multiplex PCR were conducted by a series of sensitivity tests. The positive clone vector was adjusted to the same initial concentration and diluted serially ten-fold (10 5 to 10 10 copies/μL) to serve as a template in the optimized multiplex PCR. ( D1 ). The detection limits of WMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D2 ). The detection limits of CMV;1–6 stand for 10 5 to 10 0 copies/μL. ( D3 ). The detection limits of ZYMV; 1–6 stand for 10 5 to 10 0 copies/μL ( D4 ). The detection limits of SqMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D5 ). The detection limits of TMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D6 ). The detection limits of PRSV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D7 ). The detection limits of MYSV; 1–6 stand for 10 5 to 10 0 copies/μL. ”–“represents deionized water as control. M: DNA marker (100 bp–2000 bp).

Journal: Microorganisms

Article Title: One-Step Multiplex RT-PCR Method for Detection of Melon Viruses

doi: 10.3390/microorganisms12112337

Figure Lengend Snippet: ( A ). The specificity analysis results of the multiplex RT-PCR detection method. The detection samples corresponding to lanes 1 to 7 only contain WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV, respectively. ( B ). The sensitivity tests of the multiplex RT-PCR detection method. The drop-out experiments were carried out to test the specificity of this multiplex RT-PCR, in which one pair was removed at a time to see whether the rest of the primers had cross-reacted.; lane 1: healthy plant (negative control); lane 2: detection of MYSV; lane 3: detection of PRSV; lane 4: detection of TMV; lane 5: detection of SqMV; lane 6: detection of ZYMV; lane 7: detection of CMV. ( C1 ). Detection results of using different Mg 2+ concentrations in multiplex RT-PCR amplification system. lane 1: at 1.0 mol/L; lane 2: at 1.5 mmol/L; lane 3: at 2.0 mmol/L; lane 4: at 2.5 mmol/L; lane 5: at 3.0 mmol/L; lane 6: at 3.5 mmol/L; lane 7: at 4.0 mmol/L. ( C2 ). Detection results of using different template cDNA volumes in multiplex RT-PCR amplification system. lane 1: at 0.5 μL; lane 2: at 0.75 μL; lane 3: at 1 μL; lane 4: at 1.25 μL; lane 5: at 1.5 μL; lane 6: at 1.75 μL; lane 7: at 2 μL. ( C3 ). Detection results of using different primer amounts in a multiplex RT-PCR amplification system; lane 1: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 2: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 3: at 2 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 4: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1; lane 5: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 2:2:2:1:1:1:1; lane 6: at 5 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 10:9:8:8:5:5:3:3; lane 7: at 7 × 10 −5 μmol, and molar ratios of primers for WMV, CMV, ZYMV, SqMV, TMV, PRSV, and MYSV is 1:1:1:1:1:1:1. ( C4 ). Detection results of using different dNTP concentrations in multiplex RT-PCR amplification system; lane 1: at 0.2 mmol/L; lane 2: at 0.4 mmol/L; lane 3: at 0.6 mmol/L; lane 4: at 0.8 mmol/L; lane 5: at 1.0 mmol/L; lane 6: at 1.2 mmol/L; lane 7: at 1.4 mmol/L; ( C5 ). Detection results of different amounts of Taq DNA polymerase in multiplex RT-PCR amplification system; lane 1: at 0.25 U; lane 2: at 0.5 U; lane 3: at 0.75 U; lane 4: at 1.0 U; lane 5: at 1.25 U; lane 6: at 1.5 U; lane 7: at 1.75 U. ( C6 ). Detection results of using different annealing temperatures in multiplex RT-PCR method; lane 1: at 50 °C; lane 2: at 51 °C; lane 3: at 52 °C; lane 4: at 53 °C; lane 5: at 54 °C; lane 6: at 55 °C; lane 7: at 56 °C. ( D ). The detection limits of the multiplex RT-PCR assays. The detection limits of this multiplex PCR were conducted by a series of sensitivity tests. The positive clone vector was adjusted to the same initial concentration and diluted serially ten-fold (10 5 to 10 10 copies/μL) to serve as a template in the optimized multiplex PCR. ( D1 ). The detection limits of WMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D2 ). The detection limits of CMV;1–6 stand for 10 5 to 10 0 copies/μL. ( D3 ). The detection limits of ZYMV; 1–6 stand for 10 5 to 10 0 copies/μL ( D4 ). The detection limits of SqMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D5 ). The detection limits of TMV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D6 ). The detection limits of PRSV; 1–6 stand for 10 5 to 10 0 copies/μL. ( D7 ). The detection limits of MYSV; 1–6 stand for 10 5 to 10 0 copies/μL. ”–“represents deionized water as control. M: DNA marker (100 bp–2000 bp).

Article Snippet: Using RevertAid TM first-strand cDNA synthesis kits (Dingguo Changsheng Co., Ltd., Beijing, China), the first-strand cDNA of seven viruses were synthesized following the manufacturer’s instruction.

Techniques: Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Negative Control, Amplification, Plasmid Preparation, Concentration Assay, Control, Marker